FastQCFastQC Report
Tue 28 Nov 2017
SRR491365_ccRCC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491365_ccRCC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3007269
Sequences flagged as poor quality0
Sequence length35
%GC62

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAA403.2542084E-729.00385715
CTTAAGT255.1547703E-429.0014361
CGTATAT255.1547703E-429.0014361
CGTAAGT452.8197974E-829.0014361
ACTTTCT255.1547703E-429.0014361
ACGAACT255.1547703E-429.0014361
ACGAAAT255.1547703E-429.0014361
AAGTAGT200.006074896529.0014341
CGACGTT200.006074896529.0014341
CGGTTCT200.006074896529.0014341
TGTTACT200.006074896529.0014341
ACATGTT200.006074896529.0014341
TTACGAT200.006074896529.0014341
CACAAAT200.006074896529.0014341
CCGCACT403.2565913E-729.0014341
TCGATTA1400.028.99998516
AAATTAG200.00607638428.9999813
GCTATAA353.778925E-628.99901617
ATATTAG452.8225259E-828.99852814
TAGAATA403.25992E-728.99804519
Produced by FastQC (version 0.11.2)