FastQCFastQC Report
Tue 28 Nov 2017
SRR491364_ccRCC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491364_ccRCC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4445364
Sequences flagged as poor quality0
Sequence length35
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGT502.4392648E-929.0147341
GAATAGT304.3868502E-529.0147341
CAACAAT255.141351E-429.0147341
CATTATT1250.029.0147341
TTAAGGT700.029.0147341
AACGAAT255.141351E-429.0147341
TCGTACT304.3868502E-529.0147341
TACTATT255.141351E-429.0147341
TTACGGT601.8189894E-1129.0147341
ACCAATT255.141351E-429.0147341
CCAAACT304.3868502E-529.0147341
ATTTGAT255.141351E-429.0147341
GCATATT255.141351E-429.0147341
GACTTAT403.2441494E-729.0147321
AAACACT200.00606161529.0147321
CGGCTAT452.807792E-829.0147321
TTACGAT200.00606161529.0147321
ACTTTAT200.00606161529.0147321
CAATCTT200.00606161529.0147321
TCGAACT403.2441494E-729.0147321
Produced by FastQC (version 0.11.2)