FastQCFastQC Report
Tue 28 Nov 2017
SRR491363_ccRCC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491363_ccRCC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6381213
Sequences flagged as poor quality0
Sequence length35
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAAT200.00606264329.013921
CCTACGT353.7647133E-629.0139181
AGATAAT255.1424484E-429.0139161
TAACGCT452.8090653E-829.0139161
ATTTTAT255.1588175E-428.99816713
TAACTTA255.1588175E-428.99816713
AATAAAG304.4042677E-528.99816713
TATATTG200.006078981328.9979382
TCATAAA353.7815917E-628.9972539
TTTTATT200.006081084728.99588814
TTCTTTT255.1611935E-428.99588414
TTTCAAG200.00608178628.99520512
TTAATCA304.40805E-528.9945183
GCAACAA200.006083422328.9936076
CTCGAAA403.265268E-728.9936076
TTTACGT200.00608459128.9924685
TGTTTTA1050.028.9924645
CAAGTTA200.0060869328.99018515
CCTTTAA8300.028.81939911
CCGGCTA62700.028.8095443