FastQCFastQC Report
Tue 28 Nov 2017
SRR491359_ccRCC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491359_ccRCC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3546232
Sequences flagged as poor quality0
Sequence length35
%GC61

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCAAACGCCTTGGCCGTACAGCAGCAACGAGC42040.1185483634460464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCAAG7500.029.0213991
AAGCTTA304.3797434E-529.0213971
CGTATAA950.029.0213951
CAACGGC255.1342446E-429.0213951
CTATACA200.00605466129.0213951
ACCCTAT255.1342446E-429.0213951
TACGCGG200.00605466129.0213951
GTCACGA200.00605466129.0213951
TATAGAG255.1342446E-429.0213951
TAAGCGG200.00605466129.0213951
TAATGAG255.1342446E-429.0213951
AATACGG200.00605466129.0213951
TACACGG255.1342446E-429.0213951
AAGTCAA1350.029.0213951
ATTACGT255.146556E-429.00952121
CACCTTA1450.029.00952126
AAAAACG200.006067195429.00911127
GTGTTTA850.029.00583820
ACTAATT200.006075144729.00133729
TTTTCGA1000.028.99684117
Produced by FastQC (version 0.11.2)