FastQCFastQC Report
Tue 28 Nov 2017
SRR491357_ccRCC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491357_ccRCC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4587786
Sequences flagged as poor quality0
Sequence length35
%GC62

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATT200.00600615929.06938626
CACGTTA255.0879986E-429.06651523
ATAGCTT255.0935603E-429.0610922
TCGCGTT200.006014541329.0610922
ATCGATA304.4037806E-528.99837915
CACAAAT403.2777825E-728.98061
ATTACTA1500.028.96601912
TTAATTG200.006111607428.96601312
TTTTTAT403.2922617E-728.96601312
AGTCATT403.29288E-728.9653825
CTTGCGT353.8138005E-628.9653825
TCGTTGA304.438438E-528.9650653
CCGTTGT353.814126E-628.9650653
TATAAAC800.028.96506311
TTCGTTG353.816076E-628.9631632
ATCGTTG255.195188E-428.9631632
CCGGCTA126200.028.9076843
TCGGTAG180050.028.85958316
CCGGATA51550.028.8245933
CCCGGCT126500.028.7914472
Produced by FastQC (version 0.11.2)