FastQCFastQC Report
Tue 28 Nov 2017
SRR491356_ccRCC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491356_ccRCC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337635
Sequences flagged as poor quality0
Sequence length35
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGGTGGTATAGTGGTGAGCATAGCCGCCTTGG42210.3155569344402621No Hit
GGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGTA13640.10197101600959904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTAA353.3311208E-629.4703317
GTTAGGT303.9424405E-529.47032717
ATAGGCT900.029.4612415
ATGTAGG254.699856E-429.4612415
GACATGG254.699856E-429.4612415
ATAGTGA254.699856E-429.4612415
TCGGTTT452.4176188E-829.4612415
ACGTTGA700.029.46123915
TCGATTA1600.029.46123915
TATAATT200.00562429529.46123915
CCAATTA353.3461602E-629.45329316
CGATTAA200.00563171729.45329316
TGTTGCC452.4239853E-829.45329316
ACGTGGT800.029.45329316
TGTTAGA200.00563171729.45329316
GGTAACT254.707287E-429.4532916
TGGTCAT41650.029.35513915
TAGAATG402.9327202E-729.34810618
TAGATTT304.055829E-529.34810418
GTATCAT27700.029.3107417
Produced by FastQC (version 0.11.2)