FastQCFastQC Report
Tue 28 Nov 2017
SRR491351_ccRCC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491351_ccRCC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964213
Sequences flagged as poor quality0
Sequence length50
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTTG254.3041553E-544.23606543
AGATCTT502.5465852E-1144.23606542
AGATTTT351.3861973E-744.23606542
GAGAAAT408.039933E-944.16448237
ATGAGAA351.4184297E-744.10692226
TCACGTT600.044.10692222
AACACAT408.134521E-944.10692222
GTAATTG207.8004337E-444.0632841
AAGATTT302.502411E-644.0632841
ATCTTTT650.044.06098644
CCCTCGT207.802434E-444.0609844
AATTCGT254.40612E-544.0609844
AAATCGA502.7284841E-1144.05639613
ATTAGCA254.40883E-544.05639616
CCCAACA254.40883E-544.05639614
TACGACA254.40883E-544.05639614
CTCATTG850.044.05639618
CGATCGT254.40883E-544.05639613
TGTCGGT502.7284841E-1144.05639613
TCGGTAG6950.044.05639616
Produced by FastQC (version 0.11.2)