FastQCFastQC Report
Tue 28 Nov 2017
SRR491349_ccRCC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491349_ccRCC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262756
Sequences flagged as poor quality0
Sequence length35
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATTA304.013599E-529.394516
ACAGTTA551.8189894E-1029.39449916
CGATTAA254.7637406E-429.39449916
GCTGTTA254.7637406E-429.39449916
ATAGTTT200.005687827229.39449716
TCCAATT304.01672E-529.39115115
TCTATGT254.7669196E-429.3911515
GTATCAT25250.029.33481617
ACATTTG304.178655E-529.2213623
ATGTTGA71150.029.14329715
ACTCGCA200.005937194529.13752614
GATTCAT200.00604078929.03455222
TCACGTA452.7885108E-829.033921
TGTTGAC64450.029.0068316
TGGTCAT10650.028.97718815
TGGTTAT37950.028.9651915
ATCACGT25450.028.94246920
TATCATG24750.028.94051618
ATCATGC24100.028.92738219
TCGGTAG43800.028.92142915
Produced by FastQC (version 0.11.2)