FastQCFastQC Report
Tue 28 Nov 2017
SRR491348_ccRCC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491348_ccRCC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706032
Sequences flagged as poor quality0
Sequence length35
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGTA20020.11734832640888329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA750.029.5014117
GTTAAAC200.00558740429.50140817
GCTATGC254.6713316E-429.49247716
GCTATAA402.8101385E-729.49247716
CAGATAT200.005595678529.49247716
GAGTTGT402.8101385E-729.49247716
TCTCAGA200.005595678529.49247716
GGCTATT303.9253595E-529.489815
ATGTATG200.005598162829.48979815
TAGATTT200.00576405429.31411618
GAGTTAT304.0886887E-529.31411618
CATTCTG402.9751754E-729.30089228
AGAAACA1300.029.24372714
ATCTTAG200.005832173429.24372714
TGGTTAT43500.029.15083715
ACGTTTG200.005964222429.11004323
ATCCGGC255.0422834E-429.11004323
CAAGAAG200.005964222429.11004325
TGGTCAT36150.029.08191915
GGTCATC33800.029.01256816
Produced by FastQC (version 0.11.2)