FastQCFastQC Report
Tue 28 Nov 2017
SRR491347_NRC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491347_NRC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934372
Sequences flagged as poor quality0
Sequence length50
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTGGCCGTACAGCCGCCGATGGCAGGAATGCCGAGACCGATCTCGT13570.14523123552503714Illumina Paired End PCR Primer 2 (100% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTCT302.4329383E-644.2436943
GATATTT302.4329383E-644.2436943
CGGTCTT600.044.2436942
GATCTTC207.6449045E-444.24368743
GGTCTCT207.6449045E-444.24368743
CCATTTT207.6449045E-444.24368742
CCGCTTT408.1508915E-944.0961941
CAGCTCT207.771677E-444.0961941
ACCTCCT351.4337456E-744.0464644
ATCTTTT950.044.0464644
AATTCGT408.232746E-944.0464644
AACTCGT1950.044.0464644
CTCTCTT254.4146E-544.0464644
ATTCTGT207.8149926E-444.0464644
ATTTCCT207.8149926E-444.0464644
TTCTCGT207.8149926E-444.0464644
ACAAAGC302.5107656E-644.0417317
TGTATAG502.7284841E-1144.0417314
AGGAACA1000.044.0417315
TATTGGC254.4174005E-544.0417315
Produced by FastQC (version 0.11.2)