FastQCFastQC Report
Tue 28 Nov 2017
SRR491346_NRC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491346_NRC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282817
Sequences flagged as poor quality0
Sequence length50
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTGGCCGTACAGCAGGGTGTGGCAGGAAAGCCGAGACCGATCTCGT17780.13860121903591863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTTC407.967174E-944.21284542
CATTCTC207.67229E-444.21284543
AGTATTT207.67229E-444.21284542
TATCTTT207.67229E-444.21284543
CGACAGA351.4157922E-744.1193238
TTATCTC351.4223406E-744.09341422
GATTCAA351.4223406E-744.09341423
ATACAAG700.044.09341423
ATTAGGC254.3878455E-544.0934124
CATACAA750.044.0934122
GAGCAAG600.044.0934122
TCTTTCG207.7751855E-444.0934123
TGCACGA600.044.0934124
CCTTACT454.6929927E-1044.09168628
GACAGCT302.4993824E-644.0727139
ACAGGTT302.4993824E-644.0727140
AGCGATA254.400029E-544.07270440
GAACGTT207.793192E-444.07270439
GCGATAT254.400029E-544.07270441
ACCAATT1300.044.07270441
Produced by FastQC (version 0.11.2)