FastQCFastQC Report
Tue 28 Nov 2017
SRR491345_NRC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491345_NRC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507777
Sequences flagged as poor quality0
Sequence length50
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGT254.3247714E-544.1952344
ATTTTTT551.8189894E-1244.19522544
ATCTTTT551.8189894E-1244.19522544
AACTCTT207.6829427E-444.19522544
CGATTTA302.452467E-644.1864842
CCATCTT254.32983E-544.18647842
GATTTAG254.3323613E-544.18210643
TATTTTG207.694189E-444.18210243
CATCTTG207.694189E-444.18210243
GAACAAT207.720481E-444.15151639
GCCCGCT302.4658657E-644.15151639
CGCGACA408.29823E-943.9992136
AGAACAA207.864456E-443.98620238
AACAGTC454.820322E-1043.9732118
TCGGTAG900.043.9732116
GGCTATG454.820322E-1043.9732116
TCTTGTG408.341885E-943.97320616
TTGTGTC207.8759127E-443.97320618
ATTTCCC207.8759127E-443.97320616
CTAAGGT302.5372374E-643.968881
Produced by FastQC (version 0.11.2)