FastQCFastQC Report
Tue 28 Nov 2017
SRR491344_NRC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491344_NRC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138510
Sequences flagged as poor quality0
Sequence length50
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTGGCCGTACAGCAGAAGGGGGCAGGAATGCCGCGACCGATCTCGT2980.21514692079994224Illumina Paired End PCR Primer 2 (96% over 25bp)
CGCCTTGGCCGTACAGCAGAAGGGGGCAGGAATGCCGAGACCGATATCGT2880.2079272254710851Illumina Paired End PCR Primer 2 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAAT254.307872E-544.1960140
AAAGATC254.307872E-544.1960140
CCTATTT207.6627644E-444.19600341
AAGATCT207.6627644E-444.19600341
AACTATC207.6627644E-444.19600340
CCGAGCT207.6627644E-444.19600341
ACCTATT207.6627644E-444.19600340
ACGGTTT207.6627644E-444.19600341
AACGGTT207.6627644E-444.19600340
ACTATCT207.6627644E-444.19600341
CGATATT351.3924182E-744.16394842
AGATATC254.3263834E-544.16394842
CTATTTC207.6902186E-444.16394442
ATCTCTT1300.044.14793444
ATCTCTG351.3964018E-744.14793444
AATTCGT302.4541405E-644.14793444
ATCTAGT502.5465852E-1144.14793444
ATCTAGG207.7039754E-444.14793444
CTCTCGT800.044.14793444
CTCTCGG254.3356635E-544.14793444
Produced by FastQC (version 0.11.2)