FastQCFastQC Report
Tue 28 Nov 2017
SRR491343_NRC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491343_NRC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386683
Sequences flagged as poor quality0
Sequence length50
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT207.7269203E-444.14137342
ATTTCGT4100.044.13564744
ATATCTT502.7284841E-1144.13564344
ATTTTTT351.4082434E-744.13564344
ATCTTTT254.3574146E-544.13564344
ATCTTGC207.731863E-444.13564344
GTTTTGT302.470435E-644.13564344
AACTCGG351.4082434E-744.13564344
CTCTCGT2450.044.1356444
ACAGATT551.8189894E-1244.12991340
CAGCTCT207.736808E-444.12991341
GACAGCT207.7665306E-444.0955839
TAATACA302.5261488E-643.99288618
AGCTATT302.5261488E-643.99288619
GTCATCA254.4415778E-543.9928818
GTCTCAT254.4415778E-543.9928818
TCTCATG254.4415778E-543.9928819
CATGCAG408.309144E-943.98719422
TCATTCA302.5284007E-643.98719421
CTATCAA254.444972E-543.98719421
Produced by FastQC (version 0.11.2)