FastQCFastQC Report
Tue 28 Nov 2017
SRR491342_NRC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491342_NRC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160158
Sequences flagged as poor quality0
Sequence length35
%GC63

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCT502.4483597E-929.0013481
TAGAAGT304.3996377E-529.0013481
GCAAAGT304.3996377E-529.0013481
TTCAAGT2800.029.0013481
TGTGACT255.154388E-429.0013481
CTACGCT304.3996377E-529.0013481
CGCAGAT353.7759091E-629.0013481
TACTACT255.154388E-429.0013481
CACACGT353.7759091E-629.0013481
ACCAAGA304.3996377E-529.0013481
CGTTTAT255.154388E-429.0013481
GGCTACT304.3996377E-529.0013481
GAAGGCT353.7759091E-629.0013481
ACAACGT200.00607461229.0013471
ATTAGGT200.00607461229.0013471
GTATTCT200.00607461229.0013471
CAACGAT200.00607461229.0013471
TGAAGGT552.1282176E-1029.0013471
CCCTAAT200.00607461229.0013471
GACGTCT200.00607461229.0013471