FastQCFastQC Report
Tue 28 Nov 2017
SRR491341_NRC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491341_NRC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42357
Sequences flagged as poor quality0
Sequence length35
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTGGCCGTACAGCAGGGGCTTGCAGGAATGC39599.34674315933612No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT2310.5453644025780863No Hit
ACTGCCCATAGAGAGGCGCCTTGGCCGTACAGCAG1260.2974714923153198No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCC640.1510966310173053No Hit
CGCCTTGGCCGTACAGCAGGGGCTTGCAGGAATGG490.11568335812262437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCATG900.029.11442422
ATGGGAC200.00589472229.11442226
GCAAGAC254.955606E-429.11442225
CTGGGGT200.00589472229.11442222
TATCGGG254.955606E-429.11442222
GAGCATC200.00589472229.11442222
TCACGTT304.18635E-529.11442222
CATGGGA403.024561E-729.11442225
AGCATGG452.613342E-829.07976323
AGCATGA452.613342E-829.07976323
TGAGACT200.00592921329.07976227
CACGTTC304.2206244E-529.07976223
AGCATCA200.00592921329.07976223
GAGACTC200.00592921329.07976228
TGGTTCA452.6439011E-829.0451878
GCATGAG452.6439011E-829.04518724
ATCACGT452.6439011E-829.04518721
ATCCTCT255.025365E-429.0451855
GGAGACG200.00596386429.0451858
GGATGAT200.00596386429.0451855
Produced by FastQC (version 0.11.2)