FastQCFastQC Report
Tue 28 Nov 2017
SRR491340_NRC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491340_NRC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068271
Sequences flagged as poor quality0
Sequence length35
%GC61

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGA200.005987969729.08668525
TACGCGA1100.029.00114316
TATAGTT2250.029.00114316
CTAGAAG353.776011E-629.0011416
CATAGTT200.006074761529.0011416
AAGAATA200.006079090828.99691415
TATTAGC403.260211E-728.99691415
ACTAGAA353.780302E-628.99691215
GCAACTG200.00608342328.99268717
ATAGTTA651.8189894E-1228.99268517
CTATCGA452.8310751E-828.9884621
AAATCGT200.00608920228.98705113
TTGTATT452.8323484E-828.98705113
CGATTTT304.4143664E-528.98705113
AAAACGG850.028.98705113
CGAGCTT304.4143664E-528.98705113
TTACGGA353.7903283E-628.98705113
AAGGCTT304.4143664E-528.98705113
TTTAAAC255.1692076E-428.9870513
TCAAAAG200.006091370728.98493814
Produced by FastQC (version 0.11.2)