FastQCFastQC Report
Tue 28 Nov 2017
SRR491339_NRC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491339_NRC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551323
Sequences flagged as poor quality0
Sequence length35
%GC64

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATT255.0620764E-429.09166526
AATTTTC255.081453E-429.07268322
CGACGAT255.1672687E-428.989621
CATTATT8450.028.989621
AGTTCTT255.1672687E-428.989621
CGTAAGT255.1672687E-428.989621
ACTATAT304.4125856E-528.9896181
GTACTAT200.006087150428.9896181
GATTCTT200.006087150428.9896181
GCATTAT353.7887276E-628.9896161
CACTAGT353.7887276E-628.9896161
TAAAGTT200.006095146328.9818316
ATTTCCG850.028.98182916
ACAGTTG403.2770185E-728.981012
TGAGTTT255.176258E-428.9810117
ATTATTA8500.028.981012
TACGCTG601.8189894E-1128.9810092
CTTCTTT200.00610483728.972413
CTCGTTA1650.028.9724063
TCTAGAA304.4304932E-528.9724063
Produced by FastQC (version 0.11.2)