FastQCFastQC Report
Tue 28 Nov 2017
SRR491338_NRC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491338_NRC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783565
Sequences flagged as poor quality0
Sequence length35
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGTA28780.1613622155626512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAA651.8189894E-1229.55786717
TTTAGTT304.037624E-529.36827515
CTAGAAG551.8371793E-1029.36827515
ACAGTTA651.8189894E-1229.36827516
GACGTAG254.788286E-429.36827515
TCTTATG304.037624E-529.36827515
TCGATTA1950.029.36827515
AAAACTG2250.029.36827515
TCGGATT353.4232253E-629.36827515
TCGTGAA254.788286E-429.36827516
TACTGTT200.005712359729.36827515
GGAACGT402.9160583E-729.36827515
ACTAATA402.9160583E-729.36827515
TATAGAT200.005712359729.36827515
ATAGTAA254.788286E-429.36827516
TATACGT200.005712359729.36827515
CCCGGAA502.1409505E-929.36827516
AAGATCC200.00581798429.25837726
ACATTTG200.00582863129.24743323
GGCTTGC200.00595593129.118414
Produced by FastQC (version 0.11.2)