FastQCFastQC Report
Tue 28 Nov 2017
SRR491337_NRC.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491337_NRC.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2359380
Sequences flagged as poor quality0
Sequence length35
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGTA58080.24616636574015205No Hit
TCTACGGCCATACCACCCTGAACGCGCCCGATCTC26300.1114699624477617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAA700.029.557317
GTATCAT143150.029.340517
GGTATCA154000.029.24828316
CTATCGA1200.029.19263320
TGGTATC156250.029.18570915
GGCTATG3500.029.12290615
ACGTTTG200.005989399729.08541723
TATCATG128300.029.06951118
TGGTTAT59000.029.01917515
ATCATGC122400.029.01860819
CGAGTTT502.4556357E-928.9952213
AATCGTT255.16089E-428.9952213
AATCGGG700.028.9952213
ATATTGG3750.028.99521813
TGGTCTG4650.028.92165817
TCGGTAG86900.028.88199815
ATCGGTT40400.028.87660414
TGGTCAT20800.028.8347615
TTGTCTA750.028.81648612
TTACGGA602.0008883E-1128.81648612
Produced by FastQC (version 0.11.2)