FastQCFastQC Report
Wed 6 Dec 2017
SRR330909_NN.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR330909_NN.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815217
Sequences flagged as poor quality0
Sequence length36
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGG200.00510858130.0478046
ACTAGTG200.005109027530.0472667
CAGTTGC501.6789272E-930.0445795
CGTTACA200.005111262730.0445795
CAACGGG254.1930063E-430.0424293
TCCCAAC254.1930063E-430.0424293
ATACGAA200.00514173630.00807410
AGTACAG200.005142185430.0075389
ACATAAC651.8189894E-1230.00593211
GGCAGTT303.4879064E-530.0059311
CATAGGC6900.030.00110815
GTAAAGT452.0279913E-830.00110815
CCGTTAC25550.029.9847134
CGTTACC25500.029.9267585
ACTACAG58450.029.9171458
ATCGTTA601.2732926E-1129.91667217
CACTAAC303.5595585E-529.91667217
AGCTCGA700.029.91667217
GAGGTAG405000.029.882512
TCAGTGC70350.029.87631