FastQCFastQC Report
Wed 6 Dec 2017
SRR330907_NN.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR330907_NN.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12495905
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG501.6552804E-930.086451
TTCACAG211950.029.9870871
TGAGGTA2400950.029.9811881
ATACGCG200.005172482729.97230714
CTAGCGT200.005172482729.97230712
ATAAGAC551.4551915E-1029.97230512
CGTTACC22950.029.968235
CCATCGA352.9266957E-629.967512
TCCTTCA89100.029.9344981
TGTAAAC194400.029.9316861
TCAGTGC207600.029.9052941
CCGTTAC23000.029.9030824
TTCAAGT146700.029.901871
TAATACT137750.029.889881
ACTGGAC88450.029.8823591
CCTACAC31700.029.87763810
TATAGTT1348200.029.86661116
AAGTTCC98050.029.86531813
TTGTGTG304050.029.86387313
ATAGTTA1288300.029.8570817