FastQCFastQC Report
Wed 6 Dec 2017
SRR330866_PP.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR330866_PP.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19806786
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAC83750.029.870774
TGAGGTA2760900.029.8665541
TATAGTT1610050.029.85455316
ATAGTTA1529050.029.84671617
TCAGTGC154650.029.8402021
TAGTAGG2014400.029.8368426
CGTTACC83600.029.8340385
AGTAGGT2015200.029.8339217
TAGGTTG2014450.029.8288869
GTAGGTT2015250.029.827468
ATTGGTG643300.029.8254363
GAGGTAG2777200.029.8213162
GTCTACG80350.029.8184031
TACCCTG177850.029.7942331
AGGTTGT2018650.029.79133410
CCATAGG60000.029.77995314
GTTGTGT348400.029.77654812
GTAGATT616850.029.7687268
TGTAAAC280000.029.7640081
AAGTTCC134350.029.75644513