FastQCFastQC Report
Wed 6 Dec 2017
SRR330859_PP.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR330859_PP.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11922572
Sequences flagged as poor quality0
Sequence length36
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCTA200.005164555729.98172
TACGCGT452.0421794E-829.9814516
ATACGAC254.2441353E-429.9813273
TGTGCGC303.508441E-529.9813256
CGTTAGT651.8189894E-1229.9813255
ACCGAGT303.5086417E-529.98107510
TCAGTGC99250.029.907781
TAGCTTA854550.029.8970281
TATAGTT425600.029.86873816
CCGTTAC37750.029.8621944
CGTTACC37650.029.8618775
GTTGACA517150.029.85866518
TGAGGTA709650.029.855311
ATAGTTA410350.029.83127217
CAGACTG865300.029.8272659
TGTTGAC554400.029.8197617
TAGTAGG429850.029.7999826
ATGTTGA858150.029.79453516
CGACTGG72250.029.7943413
GATGTTG859450.029.78559915
Produced by FastQC (version 0.11.2)