FastQCFastQC Report
Wed 6 Dec 2017
SRR330857_PP.fastq.gz.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR330857_PP.fastq.gz.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173425
Sequences flagged as poor quality0
Sequence length36
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77050.3545095874023718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGATT1100.030.15430626
GTGACAC200.005020707430.15430526
GCATAAG200.00504529230.12419724
AACGTTC303.4069963E-530.10881823
ATGCGTT254.1783694E-430.05998630
AGATTCA352.8563263E-630.0592928
CTTTCAG1750.029.964871
AGCTACA1500.029.9648691
AAGGTGC200.005181922629.9600262
AGTTATA1200.029.95518519
GCAGATA601.2732926E-1129.95103516
TAGCTAC200.00518953329.95103516
CGTGATA800.029.94896312
ATAGTGC2250.029.94896311
TCTAACC303.5330795E-529.94896111
CCGAGCG2450.029.94896111
TTGGTAC1400.029.94896112
TCAGTTG452.0621883E-829.9489612
TACCAGT1050.029.9475815
AGCGTAC254.2710835E-429.94757814
Produced by FastQC (version 0.11.2)