Basic Statistics: Overview

Basic Statistics: Main plots

**PCA sample distances**

This heatmap represents the Euclidean distances between the samples of the experiment.

MA Plots

These 2 plots represent the log2 fold change over the mean expression of raw counts (on the left) and over the mean of normalized counts(on the right)

PValue Distribution

P-value distribution of sequences: in blue are those sequences with expression greater than 5, in yellow lower than 5

Top 30 most expressed sequences

These heatmaps represent the expression data of the 30 most expressed sequences according with raw counts(left) and regularized log transformation(right)

All significant sequences (adj-pvalue below 0.1)

Expression data of the first 30 most significant sequences.

Citations

*Oasis: online analysis of small RNA deep sequencing data*.

Capece V, Garcia Vizcaino JC, Vidal R, Rahman RU, Pena Centeno T, Shomroni O, Suberviola I, Fischer A, Bonn S.

**Bioinformatics**. 2015
See online

*DESeq2*

Love MI, Huber W and Anders S (2014). “Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2.” Genome Biology, 15, pp. 550. http://dx.doi.org/10.1186/s13059-014-0550-8, http://dx.doi.org/10.1186/s13059-014-0550-8.
See online