FastQCFastQC Report
Wed 6 Dec 2017
SRR837503.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837503.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9577133
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG745780.778709035365803RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATTT207.767884E-444.10474824
AAATTTC207.767884E-444.10474825
AATCTCT207.7684846E-444.10405726
AACCCGT2700.044.0042841
TCAGTGC2700.044.0042841
TCCTTTA800.044.004281
TTCACGT302.5279787E-644.004281
TTCACCA502.7284841E-1144.004281
CCAACGG408.3164196E-944.004281
CACATTC1750.044.004281
TCCTATA3500.044.004281
ACCACTG351.4464604E-744.004281
AGAGGTA600.044.004281
AGCTACA454.802132E-1044.004281
TAACGGA207.855614E-444.004281
TAGCTTA900.044.004281
TTATTGC950.044.004281
CGCTCGG351.4464604E-744.004281
TCGAAAC408.3164196E-944.004281
TGTCAGT950.044.004281
Produced by FastQC (version 0.11.2)