FastQCFastQC Report
Wed 6 Dec 2017
SRR837502.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837502.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8350094
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG268480.3215293145202916RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAT207.797486E-444.0706322
GGCGTTC408.205461E-944.0706323
TTATCTC1200.044.0706322
TTTTCTC207.797486E-444.0706327
GTATCAG207.797486E-444.0706322
GTTTTCT302.5018653E-644.0706326
TGGAGTA900.044.0706322
AGTATTC408.205461E-944.0706325
GGGGGTA302.5018653E-644.0706322
TATCTCA1200.044.0706323
CTCAATT254.4033564E-544.0706326
GTAGTAT254.4033564E-544.0706323
GGAGTAT207.797486E-444.0706323
CGTTCTC408.205461E-944.0706325
GGGTTCT454.7293724E-1044.07036624
TAGTATT207.7977165E-444.07036624
GCGTTCT408.20728E-944.07036624
CATTGCC207.8480784E-444.012795
TTACTCC207.8480784E-444.012795
GTACCGA2150.044.012795
Produced by FastQC (version 0.11.2)