FastQCFastQC Report
Wed 6 Dec 2017
SRR837501.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837501.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000344
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG107330.35772564745909136RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGAGGGGAATTCTCGGGGGCCAAGGAACTCCA85130.28373413181955137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAT2400.044.06820328
GATACTC207.7987986E-444.06820325
GGCAATC207.7987986E-444.06820323
TTATCTC302.5022728E-644.06820322
CCGCTGA2500.044.06820327
CAATCTC207.7987986E-444.06820325
TCAATGG254.4041088E-544.06820327
GCAATCT207.7987986E-444.06820324
GGATACT207.7987986E-444.06820324
ATCAGAA3100.044.06820324
TATCTCA302.5022728E-644.06820323
TCAGAAC3100.044.06820325
TATCAGA3100.044.06820323
GATTCCT408.20728E-944.06820325
GGGATAC207.7987986E-444.06820323
TCTCAAT302.5022728E-644.06820325
CTCAATG254.4041088E-544.06820326
CGACCCG2550.044.06820323
TTATCAG3050.044.068222
CATCCCC1050.044.0196887
Produced by FastQC (version 0.11.2)