FastQCFastQC Report
Wed 6 Dec 2017
SRR837500.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837500.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47326355
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG3334890.7046581127999398RNA PCR Primer, Index 1 (96% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1465150.3095843742878572RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATT900.044.0775522
GATGGTT302.4994133E-644.0775522
GTATCAG351.4278339E-744.0775522
GGTGAAT254.39959E-544.0775522
AAGGGTA207.7918323E-444.07754523
ATCGCAT207.7918323E-444.07754523
ATTGTTA351.4279249E-744.0771824
CGTATTC800.044.07717525
GCGCATT207.7921577E-444.07717524
GACTTTC408.196366E-944.07717525
GCGTACT408.196366E-944.07717524
GCGTTCT3950.044.0771724
ACTTTCG207.7922794E-444.07703426
TATCAAT600.044.0116045
AGCGTGC351.4447323E-744.0116045
TTTACGT351.4447323E-744.0116045
ATGAAGC600.044.0116045
CTTTAGC351.4447323E-744.0116045
CTACTTA207.8494963E-444.01165
CCCTAAG850.044.01165
Produced by FastQC (version 0.11.2)