FastQCFastQC Report
Wed 6 Dec 2017
SRR837499.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837499.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54615117
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG715420.1309930362320747RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC254.3995176E-544.0776923
GTAATAC254.3995176E-544.0776923
GTATCAG1400.044.0776922
TGTAATA207.7917194E-444.07768622
CTACTCG207.79186E-444.0775325
CAATTTC254.399613E-544.07752625
TAATACT302.4994442E-644.07748824
TCGTATT207.791895E-444.07748424
CGTGAAC600.044.012955
AACATGC351.4444049E-744.012955
CGTTTGT302.5247718E-644.012955
TTTACGT750.044.0129475
GTTTAGC207.848323E-444.0129475
CACATCC3400.044.0129475
GGTCCTA254.437856E-544.0129475
GTAATGC207.848323E-444.0129475
TGCATCA1500.044.0129475
ACTCCTA3700.044.0129475
CATTTAT454.783942E-1044.0129475
GCATCGT207.848323E-444.0129475
Produced by FastQC (version 0.11.2)