FastQCFastQC Report
Wed 6 Dec 2017
SRR837498.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837498.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8001605
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG316230.3952082113525974RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTC502.7284841E-1144.07304823
CCGCTGA800.044.07304427
CAATCTC454.7293724E-1044.07304425
GGTTTTC351.4287798E-744.07304425
GTTTTCT351.4287798E-744.07304426
CATTTCT207.795362E-444.07304425
TATTCGG207.795362E-444.07304427
TGTGGTA302.5009085E-644.07304422
AATATTC207.795362E-444.07304425
ATTTCGC207.795362E-444.07304427
CTCAATT207.795362E-444.07304426
TGGCCTT207.795362E-444.07304422
CGTTCTC454.7293724E-1044.07304425
GCAATCT454.7293724E-1044.0727724
GCGTTCT454.7293724E-1044.0727724
GTACTTA23600.044.0151635
TACTTAG23600.044.0151636
CAATGTT454.783942E-1044.015166
GTTTGTC1000.044.015166
TGCATCA254.436198E-544.015165
Produced by FastQC (version 0.11.2)