FastQCFastQC Report
Wed 6 Dec 2017
SRR837497.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837497.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5110079
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG298810.5847463414949162RNA PCR Primer, Index 1 (96% over 29bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC83250.16291333265102162RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTC254.4048193E-544.06773823
TGGTACT302.502802E-644.06773824
GCGTTCT254.4048193E-544.06773824
GTTGGTA302.502802E-644.06773822
TTGGTAT302.502802E-644.06773823
GTGGTAC207.799719E-444.06773823
CGTTCTC254.4048193E-544.06773825
CTCAATT302.5029713E-644.0673126
TTTTCTG207.8000955E-444.06730727
TCAATTG254.4050743E-544.06730727
TACTTAG11550.044.0120776
CCTGCTA551.8189894E-1244.0120746
CTCCTAG454.783942E-1044.0120746
GTTCCCA551.8189894E-1244.0120746
AGTGGCT207.84842E-444.0120746
CCTTGTC207.84842E-444.0120746
ACATCCC8800.044.0120746
TCTGTTT351.4442776E-744.0120746
AGAAAGG454.783942E-1044.0120746
CCATCCC454.783942E-1044.0120746
Produced by FastQC (version 0.11.2)