FastQCFastQC Report
Wed 6 Dec 2017
SRR837496.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837496.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11579347
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1079680.9324187279299947RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATT650.044.11790526
TTGTTAT207.756553E-444.117925
ACCCGCT4400.044.117925
ATACTCT207.756553E-444.117926
GTCGAAT600.044.11713822
TATTCTG302.484523E-644.11541727
CGCTGAA1500.044.11541428
CAATTTG207.758705E-444.11541428
TCAATTT207.758705E-444.11541427
AGAACAT800.044.11541427
GAACATG800.044.11541428
ATCCTGT16500.044.010171
CTTTCAG351.444987E-744.0101661
TCCTTTA1050.044.0101661
TCCCATA351.444987E-744.0101661
TTCACGT254.4392935E-544.0101661
CCAACGG454.802132E-1044.0101661
ACGCTGC351.444987E-744.0101661
TCGATCC254.4392935E-544.0101661
AGAGGTA600.044.0101661
Produced by FastQC (version 0.11.2)