FastQCFastQC Report
Wed 6 Dec 2017
SRR837495.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837495.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22110393
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1289990.5834315111450077RNA PCR Primer, Index 1 (96% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA438720.19842252464711954RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT244860.11074430020307645RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCT207.756706E-444.117924
TTGTTAT254.3758133E-544.117925
AGTTTTC254.3758133E-544.117925
ATATTCT254.3758133E-544.117926
ATTGTTA950.044.11789724
GGAGTTT351.4175566E-744.11789723
TGGAGTA351.4177567E-744.117122
GGGGATA351.4177567E-744.117122
TCAATTT207.7587E-444.115627
CGCTGAA1400.044.11559728
CTATACG207.8495406E-444.0114481
TTTCAAG207.8495406E-444.0114481
TAATGCA207.8495406E-444.0114481
ACCCTAT207.8495406E-444.0114481
GACTGCT207.8495406E-444.0114481
TATTACA207.8495406E-444.0114481
TCCGTAC408.305506E-944.0114481
CATGGCG207.8495406E-444.0114481
CTGTCAC207.8495406E-444.0114481
TATAGCA207.8495406E-444.0114481
Produced by FastQC (version 0.11.2)