FastQCFastQC Report
Wed 6 Dec 2017
SRR837494.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837494.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11965509
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1004820.8397636907882481RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCCG132600.11081852013148794RNA PCR Primer, Index 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAC1450.044.584898
CATTGTA650.044.584896
TGGCACT254.1119743E-544.584893
TCCCAAC650.044.584893
CAGCATT650.044.584893
TGGTATA254.1119743E-544.584899
GTACCGA3100.044.584895
CATCCTA1450.044.584897
CACGTAA2150.044.584897
CACTGAG3350.044.584896
ACCGAGC3100.044.584897
CTTGGCC1100.044.584898
CGTGGCC254.1119743E-544.584896
CCACTAG254.1119743E-544.584898
TAGTAGG2000.044.584896
TACCGAG3100.044.584896
ACTTAGC1100.044.584897
ATACGAC254.1119743E-544.584893
GCGCCAC254.1119743E-544.584895
GCCACTA254.1119743E-544.584897
Produced by FastQC (version 0.11.2)