FastQCFastQC Report
Wed 6 Dec 2017
SRR837493.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837493.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10860517
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1398511.2877011287768345RNA PCR Primer, Index 1 (96% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA160730.14799479619616634RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCCG146190.13460685158910943RNA PCR Primer, Index 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT900.044.566579
GAACCGG900.044.566578
CTACAGA207.379471E-444.5665669
ATCCTCT302.316492E-644.5665669
TTGTCCG407.4342097E-944.5665669
CTGAGCA1350.044.5665668
TGGTATA207.379471E-444.5665669
TAAGCTG4250.044.5665669
ACGTAAA5250.044.5665668
CTAAGCT4300.044.5665668
CATCATG254.12194E-544.5665669
ACATCAT254.12194E-544.5665668
CCACTAG1050.044.5665668
CACCCAA254.12194E-544.5665668
ACATAAT254.12194E-544.5665668
ACCCTTG2250.044.5665668
TGCTATA207.379471E-444.5665669
TGCTACA254.12194E-544.5665669
ATGTTAA351.3091085E-744.5665668
CTTGTCT29550.044.5665668
Produced by FastQC (version 0.11.2)