FastQCFastQC Report
Wed 6 Dec 2017
SRR837492.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837492.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15825116
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG1454590.9191654582500375RNA PCR Primer, Index 1 (96% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCCG259860.16420732713744404RNA PCR Primer, Index 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCG254.1183997E-544.573218
CTGCTAT254.1183997E-544.573218
ACTATTA254.1183997E-544.573219
AAGTAAT9400.044.573214
TCTTGCT207.374158E-444.573213
TACTTGT407.4251147E-944.573216
GTACAGA1950.044.573215
CTCAAAG502.3646862E-1144.573217
CCCTAAG254.1183997E-544.573215
CATTGTC407.4251147E-944.573216
CAGTTGC407.4251147E-944.573218
CACATCA1600.044.573217
TTAGAAA207.374158E-444.573219
CAGCAAC254.1183997E-544.573216
TTGTCGC254.1183997E-544.573219
ACATTGT407.4251147E-944.573215
GTCTCAA502.3646862E-1144.573215
TCCTCGA207.374158E-444.573219
TTACTAT254.1183997E-544.573217
TAAGTGC254.1183997E-544.573218
Produced by FastQC (version 0.11.2)