FastQCFastQC Report
Wed 6 Dec 2017
SRR837491.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837491.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21273016
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG3226971.5169311206271832RNA PCR Primer, Index 1 (96% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT465100.2186337846970077RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCCG250030.11753387483937397RNA PCR Primer, Index 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTA254.1164094E-544.576959
AGTCCTC502.3646862E-1144.576959
TTGTGTC254.1164094E-544.576953
TTCAGTC254.1164094E-544.576953
CTGTACG10150.044.576953
ACTGATC2000.044.576957
ACTTGCC254.1164094E-544.576957
TTACTAT254.1164094E-544.576957
GTGGCTA1000.044.576957
CAACAAA254.1164094E-544.576957
GACCCTA254.1164094E-544.576958
ACCCTAA254.1164094E-544.576959
GTTACTA254.1164094E-544.576956
TGCATAT254.1164094E-544.576958
AGTTGTC254.1164094E-544.576957
GTAGACA254.1164094E-544.576959
TACTATT254.1164094E-544.576958
CAATGTT502.3646862E-1144.576956
TGCTAGA254.1164094E-544.576959
ACCGTTT1250.044.576953
Produced by FastQC (version 0.11.2)