Basic Statistics
Measure | Value |
---|---|
Filename | SRR837490.fastq.fasta.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 25847730 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 58 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG | 332325 | 1.285702845085429 | RNA PCR Primer, Index 1 (96% over 29bp) |
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT | 49537 | 0.19164932471826346 | RNA PCR Primer, Index 1 (100% over 30bp) |
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCCG | 28263 | 0.10934422481200477 | RNA PCR Primer, Index 1 (96% over 27bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTGTACA | 315 | 0.0 | 44.60136 | 3 |
GGTCGGT | 55 | 1.8189894E-12 | 44.60136 | 8 |
CCTAGTC | 60 | 0.0 | 44.60136 | 8 |
CACATAA | 230 | 0.0 | 44.60136 | 7 |
CAGCACA | 385 | 0.0 | 44.60136 | 4 |
AGTGGCT | 85 | 0.0 | 44.60136 | 6 |
ACCCTTT | 115 | 0.0 | 44.60136 | 9 |
TCCTAGT | 55 | 1.8189894E-12 | 44.60136 | 7 |
ATCAAGT | 55 | 1.8189894E-12 | 44.60136 | 4 |
TAGCACA | 60 | 0.0 | 44.60136 | 9 |
ACTAGCA | 60 | 0.0 | 44.60136 | 7 |
TGCTATA | 30 | 2.3042085E-6 | 44.60136 | 9 |
TCTTTCC | 55 | 1.8189894E-12 | 44.60136 | 3 |
ACTCCTA | 55 | 1.8189894E-12 | 44.60136 | 5 |
CTCCTAG | 55 | 1.8189894E-12 | 44.60136 | 6 |
AGTAGTT | 890 | 0.0 | 44.60136 | 7 |
ACATAAT | 230 | 0.0 | 44.60136 | 8 |
TTATTAC | 55 | 1.8189894E-12 | 44.60136 | 3 |
CATCAAG | 55 | 1.8189894E-12 | 44.60136 | 3 |
GTAGTTT | 890 | 0.0 | 44.60136 | 8 |