FastQCFastQC Report
Wed 6 Dec 2017
SRR837490.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837490.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25847730
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG3323251.285702845085429RNA PCR Primer, Index 1 (96% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT495370.19164932471826346RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCCG282630.10934422481200477RNA PCR Primer, Index 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACA3150.044.601363
GGTCGGT551.8189894E-1244.601368
CCTAGTC600.044.601368
CACATAA2300.044.601367
CAGCACA3850.044.601364
AGTGGCT850.044.601366
ACCCTTT1150.044.601369
TCCTAGT551.8189894E-1244.601367
ATCAAGT551.8189894E-1244.601364
TAGCACA600.044.601369
ACTAGCA600.044.601367
TGCTATA302.3042085E-644.601369
TCTTTCC551.8189894E-1244.601363
ACTCCTA551.8189894E-1244.601365
CTCCTAG551.8189894E-1244.601366
AGTAGTT8900.044.601367
ACATAAT2300.044.601368
TTATTAC551.8189894E-1244.601363
CATCAAG551.8189894E-1244.601363
GTAGTTT8900.044.601368
Produced by FastQC (version 0.11.2)