FastQCFastQC Report
Wed 6 Dec 2017
SRR837489.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837489.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21719583
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG2446931.12660081917779RNA PCR Primer, Index 1 (96% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT358180.1649110850793038RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAC5100.044.633338
TCCTACA5100.044.633339
ACGTAAA8450.044.633338
AGTCCTC502.3646862E-1144.6333279
TCGTTCT207.325428E-444.6333278
CCTAGTC407.3359843E-944.6333278
TCACAGA207.325428E-444.6333279
TCCGAAA302.2928562E-644.6333279
CAGTTGC351.2938654E-744.6333278
TTAGAAA207.325428E-444.6333279
TTGTCGC351.2938654E-744.6333279
TAAGTGC207.325428E-444.6333278
CGTTCTG207.325428E-444.6333279
GACCCTA407.3359843E-944.6333278
ACCCTAA407.3359843E-944.6333279
TTCCATT750.044.6333278
GTAGATT6550.044.6333278
CTAGTCC407.3359843E-944.6333279
TCGTCTC207.325428E-444.6333279
ATGACCC254.085809E-544.6333278
Produced by FastQC (version 0.11.2)