FastQCFastQC Report
Wed 6 Dec 2017
SRR837488.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837488.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13083728
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG192310.146984101167496RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGT254.3504027E-544.161281
TTTCCTT254.3504027E-544.161281
TCGAAAC254.3504027E-544.161281
TTTCATT3350.044.161281
TGTCATT3050.044.161281
ACCAATA351.4065881E-744.161281
TATGATT351.4065881E-744.161281
TGGAGTA5500.044.161281
CCCACCA254.3504027E-544.161281
CCCATAA4500.044.161281
TCAGTGC850.044.161281
AAAGCGT254.3504027E-544.161281
TCGAGGA700.044.161281
TTTAATT2000.044.161281
TGCGGGG254.3504027E-544.161281
TCCCTGA351.4065881E-744.161281
CACATTC302.4668461E-644.1612781
TAGTGCA408.059942E-944.1612781
TCCTATT551.8189894E-1244.1612781
TTCAAGT650.044.1612781
Produced by FastQC (version 0.11.2)