FastQCFastQC Report
Wed 6 Dec 2017
SRR837487.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837487.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17449322
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG428570.24560839670446796RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGCA3350.044.4812051
TTCAGTA551.8189894E-1244.4812051
TCTTATT2200.044.4812051
CAACCGT5650.044.4812051
TTCCGTA551.8189894E-1244.4812051
GTCTACG2300.044.4812051
TACGGGG207.449612E-444.4812051
AAAACCG351.3290446E-744.4812051
TATTGCG207.449612E-444.4812051
AACATTC4700.044.4812051
CGCAGCA351.3290446E-744.4812051
AACCTTC407.561539E-944.4812051
TACTAGA207.449612E-444.4812051
TAAGGGG351.3290446E-744.4812051
ACCAATA351.3290446E-744.4812051
TCGAGTA700.044.4812051
TTTGATT1400.044.4812051
TGGTGCA351.3290446E-744.4812051
TGTAAAC7350.044.4812051
CCCATCA407.561539E-944.4812051
Produced by FastQC (version 0.11.2)