FastQCFastQC Report
Wed 6 Dec 2017
SRR837486.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837486.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13691485
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG324360.23690636917763122RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCATT30250.044.308461
TTAGGTG207.5936964E-444.308461
TTAGGTA1150.044.308461
TATCAGC351.3702993E-744.308461
TCGCATT1350.044.308461
TCTTGCA2550.044.308461
AAATCGT502.5465852E-1144.308461
TGCCATT254.2657965E-544.308461
TGGACTA207.5936964E-444.308461
ACACCGT254.2657965E-544.308461
CTATACG407.825292E-944.308461
TCTTATT8150.044.308461
GTCTACG2050.044.308461
TATGCTT551.8189894E-1244.308461
CACGACC207.5936964E-444.308461
AAAACGT551.8189894E-1244.308461
TCTACTT302.4110122E-644.308461
TATTATT700.044.308461
TATTACA454.4929038E-1044.308461
CGCAGCA254.2657965E-544.308461
Produced by FastQC (version 0.11.2)