FastQCFastQC Report
Wed 6 Dec 2017
SRR837485.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837485.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18207737
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG739120.4059373221394839RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCA254.264095E-544.3115541
TGTCATT12950.044.3115541
TTAGGTA1600.044.3115541
TGGAGCA650.044.3115541
TCGCATT407.821654E-944.3115541
CCCCTAA2550.044.3115541
AAATCGT3150.044.3115541
TTGTGTA407.821654E-944.3115541
CATGCCT551.8189894E-1244.3115541
TTCAATT302.409899E-644.3115541
GTCTACG3150.044.3115541
TTTCATT14250.044.3115541
CCCACAG302.409899E-644.3115541
TATTGCG207.5911515E-444.3115541
TGTCTTA650.044.3115541
AACATTC5200.044.3115541
CCCTTAA502.5465852E-1144.3115541
TAAGGTA502.5465852E-1144.3115541
TACTAGA254.264095E-544.3115541
TTTGATT2350.044.3115541
Produced by FastQC (version 0.11.2)