FastQCFastQC Report
Wed 6 Dec 2017
SRR837484.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837484.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17534233
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG498410.2842496731964267RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTT351.4110447E-744.1436841
TTAGGTG302.4736692E-644.1436841
TTAGGTA950.044.1436841
TATCAGC551.8189894E-1244.1436841
TGTCAAC408.087227E-944.1436841
TTAGGGG1950.044.1436841
TGGAGCA454.656613E-1044.1436841
TCGCATT502.7284841E-1144.1436841
AAATCGT2000.044.1436841
TACCCAT207.734378E-444.1436841
TCACCTA207.734378E-444.1436841
TTGTGTA207.734378E-444.1436841
TTCAGTA408.087227E-944.1436841
CATGCCT650.044.1436841
CTATACG302.4736692E-644.1436841
TTCACCA750.044.1436841
CAACCGT9100.044.1436841
TTCCGTA750.044.1436841
TTCAAGT2150.044.1436841
GTCTACG1750.044.1436841
Produced by FastQC (version 0.11.2)