FastQCFastQC Report
Wed 6 Dec 2017
SRR837483.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837483.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16800317
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG627780.3736715206028553RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCA351.3709905E-744.305721
AACGTTC407.830749E-944.305721
TTAGGTA5450.044.305721
TATCAGC1750.044.305721
TTAGGGG1800.044.305721
TCTTGCA5400.044.305721
TTCAGTA502.5465852E-1144.305721
TTTAAAC254.267395E-544.305721
GTTAGGC207.5960584E-444.305721
ACCCTAT207.5960584E-444.305721
TTTCATT37100.044.305721
AAACGGT407.830749E-944.305721
AAACGCT302.4120727E-644.305721
AATGACA4350.044.305721
TATTACA600.044.305721
CCCTTAA254.267395E-544.305721
GCCTACG207.5960584E-444.305721
GTATATC207.5960584E-444.305721
TGCTATT302.4120727E-644.305721
TATAGCA502.5465852E-1144.305721
Produced by FastQC (version 0.11.2)