FastQCFastQC Report
Wed 6 Dec 2017
SRR837482.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837482.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7302846
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG315360.4318316448135425RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTA4100.045.0920031
CTATACG206.9653254E-445.0920031
TCCTATA3300.045.0920031
TGTCTTA302.1368432E-645.0920031
TTCAAGT1350.045.0920031
AGAGGTA551.8189894E-1245.0920031
TTATTGC900.045.0920031
ACACCGT406.701157E-945.0920031
TGTCAGT302.1368432E-645.0920031
AACCCGT406.701157E-945.0920031
TCGTGTA1000.045.0920031
TATTGCG206.9653254E-445.0920031
GGATATC253.8461876E-545.0920031
CGCTAAA302.1368432E-645.0920031
TACCGCA253.8461876E-545.0920031
TCTTGCA3300.045.0920031
TATTCCA253.8461876E-545.0920031
TATTTCA206.9653254E-445.0920031
TATTACA206.9653254E-445.0920031
AACATTC2700.045.0920031
Produced by FastQC (version 0.11.2)