FastQCFastQC Report
Wed 6 Dec 2017
SRR837481.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837481.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17347475
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC4840222.7901582218737886RNA PCR Primer, Index 5 (100% over 50bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG678330.39102520683845915RNA PCR Primer, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTC206.986737E-445.0642781
GTCTACG4550.045.0642781
TATTGCG206.986737E-445.0642781
TTATTGC1600.045.0642781
CCTTGCA206.986737E-445.0642781
ATTCTAA406.7393557E-945.0642781
TATTTCA502.1827873E-1145.0642741
TCTTGCA5500.045.0642741
GGATATC1150.045.0642741
TAACGGA253.8604107E-545.0642741
TATTGTA453.8016879E-1045.0642741
TCGTGTA2500.045.0642741
CGCTCGG253.8604107E-545.0642741
TGTCTTA453.8016879E-1045.0642741
AATGACA2050.045.0642741
TATTACA351.1997145E-745.0642741
TCCGTAC351.1997145E-745.0642741
TATAGCA253.8604107E-545.0642741
TTTTGCA253.8604107E-545.0642741
AGAGGTA650.045.0642741