Basic Statistics
Measure | Value |
---|---|
Filename | SRR837481.fastq.fasta.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 17347475 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 57 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC | 484022 | 2.7901582218737886 | RNA PCR Primer, Index 5 (100% over 50bp) |
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG | 67833 | 0.39102520683845915 | RNA PCR Primer, Index 1 (96% over 29bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGTTTC | 20 | 6.986737E-4 | 45.064278 | 1 |
GTCTACG | 455 | 0.0 | 45.064278 | 1 |
TATTGCG | 20 | 6.986737E-4 | 45.064278 | 1 |
TTATTGC | 160 | 0.0 | 45.064278 | 1 |
CCTTGCA | 20 | 6.986737E-4 | 45.064278 | 1 |
ATTCTAA | 40 | 6.7393557E-9 | 45.064278 | 1 |
TATTTCA | 50 | 2.1827873E-11 | 45.064274 | 1 |
TCTTGCA | 550 | 0.0 | 45.064274 | 1 |
GGATATC | 115 | 0.0 | 45.064274 | 1 |
TAACGGA | 25 | 3.8604107E-5 | 45.064274 | 1 |
TATTGTA | 45 | 3.8016879E-10 | 45.064274 | 1 |
TCGTGTA | 250 | 0.0 | 45.064274 | 1 |
CGCTCGG | 25 | 3.8604107E-5 | 45.064274 | 1 |
TGTCTTA | 45 | 3.8016879E-10 | 45.064274 | 1 |
AATGACA | 205 | 0.0 | 45.064274 | 1 |
TATTACA | 35 | 1.1997145E-7 | 45.064274 | 1 |
TCCGTAC | 35 | 1.1997145E-7 | 45.064274 | 1 |
TATAGCA | 25 | 3.8604107E-5 | 45.064274 | 1 |
TTTTGCA | 25 | 3.8604107E-5 | 45.064274 | 1 |
AGAGGTA | 65 | 0.0 | 45.064274 | 1 |