FastQCFastQC Report
Wed 6 Dec 2017
SRR837480.fastq.fasta.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR837480.fastq.fasta.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15751089
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGGGCCAAGGAACTCCAG828780.5261731426950861RNA PCR Primer, Index 1 (96% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG274300.17414668915907974RNA PCR Primer, Index 6 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAGGC207.1826985E-444.81190523
GGGTAAT207.1826985E-444.81190522
TTATCTC1950.044.81190522
ATAGGAA800.044.81190523
GAACGGA207.1826985E-444.81190522
ATAGTAC253.9905557E-544.81190523
TATCTCA1950.044.81190523
ATAGATT207.1826985E-444.81190523
GGGATAC253.9905557E-544.81190523
GTAGGGA900.044.81190522
ATCAAGG207.1826985E-444.81190522
TGGGATA253.9905557E-544.81190522
TTAACAG850.044.81190522
TCGGGAA207.1826985E-444.81190523
ACTAGGA351.2538658E-744.811922
TTAGCAG3100.044.811922
TATAGAA700.044.811922
GATAGAT302.2306594E-644.811922
TGTCGAA351.2538658E-744.811922
AGGGGTA351.2543023E-744.80986821
Produced by FastQC (version 0.11.2)